How do I resuspend my IDT primers?
To make a 100 µM storage solution: Find the oligo yield information (in nmol) on the tube label or specification sheet. Multiply this number by 10. The resulting product is the amount of buffer needed, in µL, to prepare a 100 µM solution. (If yield is 9 nmol, 90 µL of buffer is needed to make a 100 µM solution.) Click to see full answer. In this manner, how do you dissolve lyophilized primers?To make a typical 100 microMolar (100X) stock concentration of primers, dissolve the primers in a volume of sterile distilled water that is 10X the amount of nmoles in the tube, using microliters of water. This value is printed on the side of the tube.Secondly, how do you resuspend Gblocks? How to resuspend, quantify, and calculate copy number Before opening the tube, spin it down in a microcentrifuge for 3–5 seconds to ensure the DNA is in the bottom of the tube. Add molecular grade water, or a buffer such as IDTE, to reach a final concentration of 10 ng/µL. Vortex briefly. Likewise, people ask, how do you dilute a primer? Use PCR-grade water (DNase- and RNase-free) to reconstitute and dilute your primers. To make a 10 μM working primer solution, follow these steps: Add 10 μL of primer stock solution to an RNase- and DNAse-free tube. Add 90 μL of PCR-grade water. Mix by vortexing. How do oligos dissolve?Dissolve the oligo in TE (10 mM Tris pH 8.0, 0.1 mM EDTA). Use the IDT Resuspension Calulator under the “SciTools®” tab of the IDT website for help determining volume. Alternatively, sterile dH2O can be used. DNA kept frozen in a nuclease-free environment should be stable for years.
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